Easy Reefs Easyroti 250

EasyRoti PRO from Easy Reefs is a bulk bag of 250 ml concentrated phytoplankton for rotifer cultures. Consists of the two main microalgae species at the correct concentration of Nannochloro ...

Easy Reefs Easyroti 250

EasyRoti PRO 250 ml Rotifer Culture Gel

EasyRoti PRO from Easy Reefs is a bulk bag of 250 ml concentrated phytoplankton for rotifer cultures. Consists of the two main microalgae species at the correct concentration of Nannochloropsis (80%) and Tetraselmis (20%).

Easybooster Composition

Nannochloropsis (80%) / Tetraselmis (20%) The special properties of each of the microalgae in this formula ensure an optimal nutritional profile for rotifers.

Dosage

The dosage depends on the number of rotifers reached. The average consumption is 4 ml EasyRoti per liter of rotiferen cultivation per day (25% of the rotifers cultivation is harvested every day).

Instructions

Follow the directions below to maintain a rotifer culture

Required material

3 cylinder flasks of 2 L with slow bubbling. Inocula for rotifers.
50 micron net

Need food

Easy Reefs EasyRoti or Nannochloropsis (80%) + Tetraselmis (20%)

Breeding cycle from strain: 5 days

Recommended sea water density: 1019, although they can be grown well up to 1022.

Growing temperature: 22ºC to 28ºC. Optimal breeding temperature: 24ºC.

To filter the rotifers, use a 50 micron net.

Once you have started the culture, you can harvest rotifers after 6 days. You have one butt for the "tribe" and another for the "production" culture.

The following information describes the dosage to be used under ideal conditions, assuming the rotifer density is ideal. Sometimes the quantities of microalgae that are added may not adapt to the demand for rotifers. This is because humans in aquariums do not count rotifers, so the amount of microalgae added is not exactly equal to the amount of rotifers. If the culture water becomes clear, it would mean that the amount of microalgae was not sufficient. If rotifer is left without food for more than 4 hours, the number of eggs will decrease and the yield of the population will also increase. It is essential that rotifers always have food.

Rotifers are very sensitive to changes in water parameters. The rotifers population would be affected if the water quality declines. If you observe excessive contamination in the cylinder, filter the rotifer and start the culture in a new clean flask and add the required amount of microalgae.

Depending on the intensity of the green color of the bottle, this indicates how much microalgae are needed. Over time and experience, the difference in green color will make us do our own feeding protocols. There is no difference between using live or Easyroti as food.

We place a glass tube with a soft air bore (5-10 bubbles per second) in the volumetric flask used for rotifer cultivation. This air bubbles ensures a homogeneous mixture in the culture. Excessive movement of the water causes rotifers to lose their eggs and this way they don't hatch.

It is recommended to feed twice a day. But due to time constraints, rotifers are usually fed once a day.

DAY 1

Initial conditions: "Sieve culture" with 500 ml seawater, number of rotifers 400 rot./mL to 600 rot./mL and 4 ml Easyroti Nannochloropsis (80%) + Tetraselmis (20%).

Final conditions: The culture will end with a volume of 1L with microalgae.

Directions for use: Resuspend 4 ml Easyroti in 0.5 L sea water. Add the rotiferation inocula and add seawater until the total volume is 1 liter.

DAY 2

Initial conditions: 1 liter of rotifer cultivation. It will be almost clear and without microalgae. Estimated number of rotifers: 500 rot / ml

Final conditions: The culture will have a content of 1.4 L with microalgae.
Directions for use: Resuspend 4 ml Easyroti in 0.5 L sea water and add 0.4 L to the rotifer culture.

DAY 3

Initial conditions: 1.4 L rotifer culture. It will be almost clear and without microalgae. Estimated number of rotifers: 500-600 rot / ml

Final conditions: The culture will have a volume of 1.8 L with microalgae.
Directions for use: Resuspend 8 ml Easyroti in 0.5 L sea water and add 0.4 L to the rotifer culture.

DAY 4

Initial conditions: 1.8 liters of rotifer cultivation. It will be almost clear and without microalgae. Estimated number of rotifers: 600 rot / ml

Final conditions: The culture will have a content of 2 L with microalgae.
Directions for use: Resuspend 6 ml Easyroti in 0.25 L sea water and add 0.2 L to the rotifer culture.

DAY 5

Initial conditions: 2 L rotifer culture. It will be almost clear and without microalgae. Estimated number of rotifers: 700-800 rot / ml

From this "strain" culture we are given a volume of 0.5 L to start a new "strain" culture in another 2 L flask and to start a new culture cycle from day 1.

We will add Easyroti to the old "stock" culture, from now on "production" culture, until it reaches 2 l. If the cylinder is very dirty, we pour the culture into a clean flask and proceed with the production culture.

Instructions

We add 8 ml Easyroti to 0.5 L sea water to the production culture. For the "tribe" culture, one starts